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. 2018 Nov 7;10(11):614. doi: 10.3390/v10110614

Table 1.

Primers used in this study.

Primer Use Sequence Primer Name
galK insertion 1 CAACGTCCACCCACCCCCGGGACAAAAAAGCCCGCCGCCCCCTTGTCCTTTCCTGTTGACAATTAATCATCGGCA UL99galK 5′
galK insertion 1 GTGTCCCATTCCCGACTCGCGAATCGTACGCGAGACCTGAAAGTTTATGAGTCAGCACTGTCCTGCTCCTT UL99galK 3′
Reversion primer CAACGTCCACCCACCCCCGGGACAAAAAAGCCCGCCGCCCCCTTGTCCTTTGTGAGCAAGGGCGAGGAGCTGTTCACCG UL99eGFP 5′
Reversion primer GTGTCCCATTCCCGACTCGCGAATCGTACGCGAGACCTGAAAGTTTATGAGTTACTTGTACAGCTCGTCCATGCCGAGAGT UL99eGFP 3′
qPCR/RTqPCR ATGGTTTTGCAGGCTTTGATG UL122 FOR
qPCR/RTqPCR ACCTGCCCTTCACGATTCC UL122 REV
RTqPCR GCCTTCCCTAAGACCACCAAT UL123 FOR
RTqPCR ATTTTCTGGGCATAAGCCATAATC UL123 REV
RTqPCR GGC GTG AAA AAC ATG CGT ATC AAC UL44 FOR
RTqPCR TAC AAC AGC GTG TCG TGC TCC G UL44 REV
RTqPCR CCCTCGGCTTCTCACAACAAT UL54 FOR
RTqPCR CGAGTTAGTCTTGGCCATGCAT UL54 REV
RTqPCR CACACAACACCGTCGTCCGATTAC UL32 FOR
RTqPCR GGTTTCTGGCTCGTGGATGTCG UL32 REV
RTqPCR GTGTCCCATTCCCGACTCG UL99 FOR
RTqPCR TTCACAACGTCCACCCACC UL99 REV
RTqPCR ACCCACTCCTCCACCTTTGAC GAPDH 2 FOR
RTqPCR CTGTTGCTGTAGCCAAATTCGT GAPDH REV
qPCR CCCCTTCCATCACATTGCA MDM2 3 FOR
qPCR AGTTTGGCTTTCTCAGAGATTTCC MDM2 REV

1 Underlined sequences correspond to the pGalK sequences. 2 GAPDH, glyceraldehyde 3-phosphate dehydrogenase. 3 MDM2, mouse double minute 2 homolog.