Table 1.
Characterization of sequence-defined probes used for Lupinus angustifolius nuclear genome library screening.
| Probe | Accession Number of Sequence Used for Primer Design | Accession Number of Probe Sequence | Tm 1 | Probe Size (bp) | Primer Pairs |
|---|---|---|---|---|---|
| La ACC | XM_003638746 | MF378602 | 58 °C | 404 | Pri-129 + Pri-131 |
| La BCCP | XM_003534455 | MF378603 | 56 °C | 251 | Pri-139 + Pri-140 |
| La CT-α | NM_001249264 | MF378604 | 58 °C | 437 | Pri-142 + Pri-145 |
| Mt BC | XM_003630560 | MF378605 | 60 °C | 1141 | J08-35 + J08-36 |
1 Tm, annealing temperature. ACC: cytosolic acetyl-coenzyme A carboxylase; BCCP: biotin carboxyl carrier protein; CT: alpha-carboxyltransferase; BC: biotin carboxylase.