. 2018 Oct 30;10(11):593. doi: 10.3390/v10110593

Table 1.

Reverse genetic systems for ZIKV, full-length infectious clones.

ZIKV Isolate	Lineage	Isolation	Cloning Strategy	Plasmid Name	Reporter	Tested	Reference
Paraiba_01/2015	Asian	Brazil, 2015	Synthetic intron insertion	ZIKV-1	-	Cell culture	[78]
				ZIKV-ICD
				ZIKV-NS3m
MR766	African	Uganda, 1947	Synthetic intron insertion		-	Cell culture	[79]
FSS13025	Asian	Cambodia, 2010	Subgenomic fragments	pFLZIKV	RLuc	Cell culture mosquito-mouse model	[80]
MR766	African	Uganda, 1947	Subgenomic fragments		-	Cell culture mouse model	[82]
H/PF/2013	Asian	French Polynesia, 2013
SPH2015	Asian	Brazil, 2015
BeH819015	Asian	Brazil, 2015
BeH819015	Asian	Brazil, 2015	Subgenomic fragments	icDNA BeH819015	nLuc	Cell culture	[83]
					GFP
					mCherry
MR766^NIID	African	Uganda 1947	Subgenomic fragments	ZIKV-MR766^NIID-MC	GFP	Cell culture	[85]
MR766^NIID	African	Uganda 1947	Subgenomic fragments	ZIKV_GFP	GFP	Cell culture	[85]
PF	Asian	French Polynesia, 2013	Subgenomic fragments	PF	-	Cell culture	[86]
DAK	African	Dakar, 1984		DAK
(MART)	Asian	Martinique, 2015		PF/DAK
				DAK/PF
				PF/MART
MR766^M	African	Uganda, 1947	Subgenomic fragments	MR766^MC	-	Cell culture	[87]
BeH819015	Asian	Brazil, 2015		BR15^MC
				CHIM
ZIKV_Natal	Asian	Natal, 2015	Subgenomic fragments			Cell culture	[89]
PRVABC59	Asian	Puerto Rico, 2015	Subgenomic fragments			Cell culture mosquito-mouse model	[90]
MR-766	African	Uganda, 1947	Subgenomic fragments	pBac/MR-766,	-	Cell culture	[81]
P6-740	Asian	Malaysia, 1966		pBac/P6-740
PRVABC-59	Asian	Puerto Rico, 2015		pBac/PRVABC-59
MR766	African	Uganda 1947	Mutational inactivation CEPs	synZIKV-MR766	RLuc	Cell culture	[91]
H/PF/2013	Asian	French Polynesia, 2013	Mutational inactivation CEPs	synZIKV-H/PF/2013	FP635	Cell culture	[91]

nLuc, nano-luciferase; RLuc, Renilla luciferase; FP635, turbo far-red fluorescent protein; icDNA, infectious cDNA clone; CEPs, cryptic E. coli promoters.