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. 2018 Nov 5;10(11):1679. doi: 10.3390/nu10111679

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(Panel A) SDS-PAGE protein profile of raw almonds submitted to chew, gastric and intestinal environments without adding enzyme mixture. Lane 1: untreated almond. (Panel B) Electrophoretic profiles of digestive fluids of almond raw (lane 1), autoclaved for 10 min (lane 2) and prehydrated and autoclaved for 10 min (lane 3). M: MW reference standard. Bands submitted to in-gel tryptic digestion for further HPLC-MS/MS analysis, were marked with letters (U1-U6 for undigested sample) and numbers (band 1a and 1b were processed as single band).