Table 1.
List of proteins identified by HPLC-MS/MS analysis followed by software data processing of selected bands in-gel digested referred to raw almond samples undigested (CTRL-NE) and digested (CTRL-GI) along with digested almond autoclaved (AC10-GI) and prehydrated autoclaved (H2O-AC10-GI). All the relevant software parameters were also included.
| Sample | Band | Accession Number | Type of Protein (Organism) | Allergen | Coverage (%) | Unique Peptides | Score |
|---|---|---|---|---|---|---|---|
| CTRL-NE | U1 | Q945K2 | (R)-mandelonitrile lyase 2 (Prunus dulcis (Mill.) D. A. Webb) | 30.37 | 18 (18) | 30.34 | |
| U2 | Q945K2 | (R)-mandelonitrile lyase 2 (Prunus dulcis (Mill.) D. A. Webb) | 11.55 | 7 (7) | 0 | ||
| U3 | Q9SW89 | Abscisic acid response protein (Prunus dulcis (Mill.) D. A. Webb) | 43.07 | 8 (8) | 19.35 | ||
| U4 | Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 12.16 | 8 (8) | 1.65 | |
| U5 | Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 8.53 | 6 (1) | 8.59 | |
| E3SH28 | Prunin 1 (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0101 | 8.17 | 6 (1) | 6.94 | ||
| U6 | Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 16.33 | 11 (2) | 21.8 | |
| E3SH28 | Prunin 1 (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0101 | 15.25 | 10 (1) | 16.56 | ||
| A7Y7K3 | Putative lipid-transfer protein (Prunus dulcis (Mill.) D. A. Webb) | 40.91 | 3 (3) | 9.77 | |||
| E3SH29 | Prunin 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0201 | 13.10 | 7 (7) | 4.43 | ||
| CTRL-GI | 1 | Q945K2 | (R)-mandelonitrile lyase 2 (Prunus dulcis (Mill.) D. A. Webb) | 36.41 | 31 (31) | 17.64 | |
| P06278 | Alpha-amylase (Bacillus licheniformis) | 9.96 | 10 (10) | 1.77 | |||
| 2 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 70.45 | 55 (28) | 100.16 | ||
| P04745 | Alpha-amylase (Homo sapiens Linnaeus) | 33.27 | 26 (4) | 58.45 | |||
| Q945K2 | (R)-mandelonitrile lyase 2 (Prunus dulcis (Mill.) D. A. Webb) | 21.31 | 12 (12) | 1.83 | |||
| 3 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 15.66 | 8 (8) | 0 | ||
| 4 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 25.83 | 21 (20) | 10.21 | ||
| Q7M3E1 | Chymotrypsin-C (Bos taurus Linnaeus) | 7.09 | 3 (2) | 3.18 | |||
| 6 | P00761 | Trypsin (Sus scrofa Linnaeus) | 22.08 | 5 (4) | 3.73 | ||
| AC10-GI | 7 | P06278 | Alpha-amylase (Bacillus licheniformis) | 3.32 | 3 (3) | 2.05 | |
| 8 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 60.86 | 47 (23) | 80.76 | ||
| 9 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 33.86 | 24 (23) | 18.86 | ||
| 10 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 19.37 | 13 (13) | 10.92 | ||
| 11 | P00766 | Chymotrypsinogen A (Bos taurus) | 15.10 | 5 (4) | 3.53 | ||
| 12 | P00761 | Trypsin (Sus scrofa Linnaeus) | 18.61 | 5 (4) | 6.36 | ||
| P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 13.50 | 10 (9) | 5.46 | |||
| H2O-AC10-GI | 14 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 50.68 | 44 (22) | 58.65 | |
| 15 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 30.14 | 20 (19) | 9.4 | ||
| 16 | P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 19.77 | 14 (14) | 9.04 | ||
| Q7M3E1 | Chymotrypsin-C (Bos taurus Linnaeus) | 7.09 | 3 (3) | 5.08 | |||
| 17 | P00766 | Chymotrypsinogen A (Bos taurus Linnaeus) | 11.02 | 3 (2) | 9.49 | ||
| 18 | P00761 | Trypsin (Sus scrofa Linnaeus) | 39.83 | 9 (9) | 11.92 | ||
| P00690 | Pancreatic alpha-amylase (Sus scrofa Linnaeus) | 12.72 | 7 (7) | 6.03 |