Table 2.
List of proteins identified by HPLC-MS/MS analysis followed by bioinformatic search via commercial software of low molecular weight fraction (<6 kDa) isolated from raw almond digest of (CTRL-GI), autoclaved (AC10-GI) and prehydrated autoclaved almond (H2O-AC-GI) along with the relevant parameters provided by software.
| Sample | Accession Number | Type of Protein (Organism) | Allergen | Coverage (%) | Unique Peptides | Score |
|---|---|---|---|---|---|---|
| CTRL-GI | C0L0I5 | Non-specific lipid-transfer protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 3, Pru du 3.0101 | 40.65 | 11 (10) | 4.11 |
| P82944 | Seed allergenic protein 1 (Fragments) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 2S Albumin | 60.71 | 5 (4) | 10.21 | |
| Q43608 | Pru2 protein (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 71.83 | 195 (7) | 297.12 | |
| E3SH28 | Prunin 1 (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0101 | 80.76 | 323 (4) | 672.17 | |
| Q8GSL5 | Profilin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 4 | 40.46 | 17 (17) | 9.7 | |
| P82952 | Seed allergenic protein 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du AP | 48.00 | 15 (15) | 39.02 | |
| Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 81.85 | 335 (14) | 697.42 | |
| Q8H2B9 | 60S acidic ribosomal protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 5, Pru du 5.0101 | 37.17 | 23 (22) | 9.42 | |
| AC10-GI | E3SH29 | Prunin 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0201 | 68.65 | 213 (4) | 363.33 |
| C0L0I5 | Non-specific lipid-transfer protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 3, Pru du 3.0101 | 32.52 | 9 (9) | 2.57 | |
| P82944 | Seed allergenic protein 1 (Fragments) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 2S Albumin | 60.71 | 5 (4) | 11.76 | |
| Q43608 | Pru2 protein (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 69.05 | 209 (6) | 359.74 | |
| E3SH28 | Prunin 1 (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0101 | 78.40 | 292 (4) | 700.81 | |
| Q8GSL5 | Profilin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 4 | 30.53 | 13 (13) | 4.48 | |
| P82952 | Seed allergenic protein 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du AP | 48.00 | 10 (10) | 17.94 | |
| Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 78.77 | 300 (10) | 712.31 | |
| Q8H2B9 | 60S acidic ribosomal protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 5, Pru du 5.0101 | 37.17 | 13 (13) | 5.05 | |
| H2O-AC10-GI | E3SH29 | Prunin 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0201 | 78.97 | 272 (16) | 630.16 |
| C0L0I5 | Non-specific lipid-transfer protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 3, Pru du 3.0101 | 27.64 | 7 (6) | 1.43 | |
| P82944 | Seed allergenic protein 1 (Fragments) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 2S Albumin | 78.57 | 6 (5) | 8.77 | |
| Q43608 | Pru2 protein (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 77.58 | 261 (10) | 592.97 | |
| E3SH28 | Prunin 1 (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6, Pru du 6.0101 | 83.30 | 321 (8) | 941.16 | |
| Q8GSL5 | Profilin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 4 | 21.37 | 10 (10) | 8.1 | |
| P82952 | Seed allergenic protein 2 (Fragment) (Prunus dulcis (Mill.) D. A. Webb) | Pru du AP | 48.00 | 9 (9) | 21.22 | |
| Q43607 | Prunin (Prunus dulcis (Mill.) D. A. Webb) | Pru du 6 | 82.94 | 320 (5) | 954.46 | |
| Q8H2B9 | 60S acidic ribosomal protein (Prunus dulcis (Mill.) D. A. Webb) | Pru du 5, Pru du 5.0101 | 37.17 | 16 (16) | 8.63 |