Figure 3.

Representative Two-dimensional gel electrophoresis maps of the control and pectolinarigenin-treated MKN28 cells. The (A) control and (B) PEC-treated (100 μM) of MKN28, the cells were incubated with the 100 µM of PEC for 24 h. The total proteins were separated on 18 cm linear IPG strips (pH 4–7) in the first dimension and in the second dimension with 12% SDS-PAGE. The gels were then silver stained. The numbered arrows indicate protein spots successfully identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI/TOF-MS). The experiments were performed in triplicate.