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Validation of differentially expressed proteins using immunoblotting. (A,B) The cell lysates prepared from Control (DMSO)—and PEC (100 μM)-treated cells were subjected to SDS-PAGE for protein separation and LRSAM1, DDX4, PI3K-β, and CIP2A proteins were detected using the corresponding antibodies, and β-actin was used as a loading control. The experiments were performed in triplicate (* p < 0.05 vs. control).
