Table 2.
Primer sequences used for RT-qPCR validation of the microarray data.
| Gene | Primer Direction | Primer Sequence |
|---|---|---|
| GAPDH | Forward | 5′-AGGTCGGTGTGAACGGATTTG-3′ |
| Reverse | 5′-TGTAGACCATGTAGTTGAGGTCA-3′ | |
| FAS | Forward | 5′-GCTGCGGAAACTTCAGGAAAT-3′ |
| Reverse | 5′-AGAGACGTGTCACTCCTGGACTT-3′ | |
| ACC1 | Forward | 5′-GCCTCTTCCTGACAAACGAG-3′ |
| Reverse | 5′-TGACTGCCGAAACATCTCTG-3′ | |
| CPT1α | Forward | 5′-ATCTGGATGGCTATGGTCAAGGTC-3′ |
| Reverse | 5′-GTGCTGTCATGCGTTGGAAGTC-3′ | |
| CPT2 | Forward | 5′-GCCTGCTGTTGCGTGACTG-3′ |
| Reverse | 5′-TGGTGGGTACGATGCTGTGC-3′ | |
| PPARα | Forward | 5′-GGCACCCTCACATCATCAAACTG-3′ |
| Reverse | 5′-TGGAACAGACGGCGGCTTTC-3′ | |
| SIRT1 | Forward | 5′-TGTGAAGTTACTGCAGGAGTGTAAA-3′ |
| Reverse | 5′-GCATAGATACCGTCTCTTGATCTGAA-3′ | |
| PGC1α | Forward | 5′-AAGTGTGGAACTCTCTGGAACTG-3′ |
| Reverse | 5′-GGGTTATCTTGGTTGGCTTTATG-3′ |
GAPDH: glyceraldehyde-3-phosphate dehydrogenase; FAS: fatty acid synthase; ACC1: acetyl-CoA; carboxylase 1; CPT1α and CPT2: carnitine palmitoyltransferase 1-alpha and 2; PPARα: peroxisome proliferator-activated receptor alpha; SIRT1: sirtuin 1; PGC1α: peroxisome proliferator-activated receptor gamma coactivator 1-alpha.