Figure 6.

Detection of the ZIKV-NS1 protein in cell culture supernatants and lyates, as well as sera of animal infection model by the DAS-ELISA. Supernatants (A,B) and cell lysates (C,D) of Vero and BHK cells infected with ZIKV from 12, 24, 36, 48, 60, and 72 h were detected by DAS-ELISA. (E,F) Sera of tree shrews infected with ZIKV from 1 to 12 days were detected by DAS-ELISA. A Real-time quantitative reverse transcription polymerase chain reaction analysis of the supernatant of Vero, BHK (G) and the sera of tree shrews (H) after infected with ZIKV. (I) Western blot assay of supernatants and cell lysates of BHK and Vero cells infected with ZIKV.