Table 1.

List of sequences and source of primers and probes used to detect human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) products with digital droplet PCR (ddPCR). LTR—long terminal repeat; Fw—forward; Rv—reverse; FAM—fluorescein amidite; BHQ1—black hole quencher 1.

Target	Sequence (5′–3′)	Reference
5′-LTR Fw	TTAAGCCTCAATAAAGCTTGCC	[43]
5′-LTR Rv	GTTCGGGCGCCACTGCTAG
5′-LTR Probe	FAM–CCAGAGTCACACAACAGACGGGCA–BHQ1
Gag Fw	CATGTTTTCAGCATTATCAGAAGGA	[44]
Gag Rv	TGCTTGATGTCCCCCCACT
Gag Probe	HEX—CCACCCCACAAGATTTAAACACCATGCTAA–BHQ1
2-LTR Fw	CTAACTAGGGAACCCACTGCT	[45]
2-LTR Rv	GTAGTTCTGCCAATCAGGGAA
2-LTR Probe	FAM—AGCCTCAATAAAGCTTGCCTTGAGTGC–BHQ1
RPP30 Fw	GATTTGGACCTGCGAGCG	[42]
RPP30 Rv	GCGGCTGTCTCCACAAGT
RPP30 Probe	FAM–CTGACCTGAAGGCTCT–BHQ1