Table 2.

Elemental combustion analysis in N. salina during reduced cultivation temperature. Elemental carbon, nitrogen, C/N, and total protein content determination for Nannochloropsis salina after a 96-h growth period: Samples were collected in triplicates (n = 3) and analyzed using SAS (p ≤ 0.05). No significant difference was observed with elemental analysis for temperature treatments. % w/w elemental analysis of carbon, nitrogen, carbon to nitrogen, and calculated total protein content with ±standard error mean (SEM) are listed in table. * Total protein contents (%w/w) were estimated using formula = %N × 4.78.

Growth Temperatures
% Composition	25 °C	15 °C	10 °C	5 °C
Carbon	41.31 ± 0.76	44.07 ± 0.41	41.88 ± 0.38	40.86 ± 1.54
Nitrogen	5.98 ± 0.06	6.59 ± 0.07	6.00 ± 0.06	5.87 ± 0.15
C/N	6.91 ± 0.09	6.69 ± 0.68	6.98 ± 0.09	6.95 ± 0.15
* Total protein	28.57 ± 0.30	31.49 ± 0.34	28.70 ± 0.29	28.05 ± 0.70