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. 2018 Nov 15;10(11):446. doi: 10.3390/cancers10110446

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Establishment of ADSC labeled with enhanced green fluorescent protein (eGFP) and combined with modified E7’ (ADSC-E7’-eGFP). (A) Schematic diagram of pLL3.7-E7’-eGFP construction; (B) agarose gel electrophoresis of plasmid pLL3.7-E7’-eGFP (M: 1 kb DNA ladder; lane 1: Undigested plasmid; lane 2: uNheI (8555 bp); lane 3: NheI and BsrGI (1636 bp and 6919 bp); lane 4: bNcoI (4554 bp, 2845 bp, and 1156 bp); lane 5: bSalI (2955 bp, 2514 bp, 2020 bp, and 1066 bp)); (C) illustration of lentiviral transduction of primary ADSCs; and (D) fluorescence microscopy and flow cytometric analysis of ADSC-E7’-eGFP cells.