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. 2018 Nov 15;10(11):446. doi: 10.3390/cancers10110446

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Evaluation of apoptosis in tumor tissues by TUNEL staining. Representative fluorescence images of (A) the CT26 tumor with different treatments; or (B) the LLC1 tumor with different treatments. Apoptotic-positive cells were shown in green (arrows) and the cellular nucleus was stained by DAPI and shown in blue. (C,D) The number of apoptotic-positive cells in the microscopic fields were calculated. The quantitative results are presented as means + standard error of means (SEM); *** indicates p < 0.001 using unpaired t-test with Welch’s correction. Magnification A,B: 50 µm.