Figure 4.

Basic regulated genes. A total of 186 genes were basically up- or down-regulated in the pairwise comparison of the DON-treatment group under low glucose concentration. The highest up- and down-regulated genes in the pairwise comparisons within the pool of basically regulated genes are shown (A). SLC7A11 (glutamate transporter) and PHB (Prohibitin) were the most frequently occurring genes in the comparisons. Therefore, SLC7A11 and PHB were analyzed via qPCR (relative quantification [%]; N = 5). Comparisons that were significantly regulated were asterisked (p < 0.01 **, p < 0.001 ***) (B), and Western blot (N = 3) analysis was performed (C). PBH showed a contrarian gradient in the expression depending on glucose consumption, DON-concentration, and application. SLC7A11 had a lower expression in DON 50 ap^high, 50 bl^high, and 200 ap^high when compared to DON 50 ap^low, 50 bl^low, and 200 ap^low.