Table 2.

Analyzed genes via qPCR (N = 5). Different genes were analyzed with qPCR, which were related to metabolic pathways: TXNIP, COX5B, GLUT1, MCT1, SGLT1, PHB and SLC7A11. All comparisons were examined under low and high glucose. Therefore, 18S and actin were used as housekeeping genes. All comparisons were examined under low and high glucose. All treatment groups (50 ap; 50 bl; 200 ap; 200 bl, 2000 ap and 2000 bl) were compared to the control [CON = 100%] and significantly regulated genes were asterisked (p < 0.05 *; p < 0.001 **; p < 0.001 ***). The values shown in the table represent relative quantification [%].

	TXNIP
	18S		Actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	109.68	112.25	138.83	128.94 *
50 bl	100.46	115.4	123.68	130.74 *
200 ap	86,65	113.29	113.81	130.13 *
200 bl	125.99	123.68	127.16	133.18 *
2000 ap	216.1 ***	127.75	110.33 *	142.08 *
2000 bl	111.03	124.26	96.82	94.17 ***
	COX5B
	18S		Actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	102.34	89.09	129.53	102.45
50 bl	105.6	92.02	127.62	104.36
200 ap	85.86	96.37	112.77	110.82
200 bl	109.18	89.09	110.19	96.03
2000 ap	242 ***	144.47	125.12 ***	158.91 ***
2000 bl	172.4	140.03 ***	149.48	105.92
	GLUT1
	18S		Actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	127.16	91.59	160.96 **	105.21
50 bl	126.58	97.72	155.83 **	110.7
200 ap	119.75	138.84	157.28 **	159.48 *
200 bl	150.18	115.4	151.57 *	124.26
2000 ap	460.01 ***	891.61 ***	237.84 ***	985.62 ***
2000 bl	688.67 ***	483.44 ***	591.46 ***	366.38 ***
	MCT1
	18S		Actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	115.94	85.46	146.75 ***	94.95
50 bl	119.27	88.27	145.4 ***	96.72
200 ap	93.74	92.99	123.11	104.56
200 bl	99.08	88.27	100	91.93
2000 ap	194.08 *	112.8	99.66	125.58
2000 bl	154.3	100	133.79 **	73.3
	SGLT1
	18S		Actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	118.65	107.14	150.18 ***	123.32
50 bl	123.11	114.87	151.57 ***	130.13
200 ap	88.68	80.8	116.47	92.08
200 bl	118.41	108.21	117.98	118.53
2000 ap	176.23 **	78.43	85.75	90.27
2000 bl	81.78	62.2	67.43 ***	46.12 ***
	PHB
	18S		β-actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	127.16	91.59	160.96	105.21
50 bl	126.58	97.72	155.83	110.7
200 ap	119.75	138.84	157.28	159.48
200 bl	150.18	115.4	151.57	124.26
2000 ap	460.01 ***	891.61 ***	237.84 ***	985.62 ***
2000 bl	688.67 ***	483.439 ***	591.46 ***	366.38 ***
	SLC7A11
	18S		β-actin
	Low	High	Low	High
CON	100	100	100	100
50 ap	127.75	110.19	161.7	126.58
50 bl	126.99	136.29	153.48	154.4 ***
200 ap	92.87	84.67	121.98	97.27
200 bl	97.72	108.17	98.62	116.47
2000 ap	30.57 ***	62.31	15.8 ***	68.87 **
2000 bl	31.59 ***	92.02	28.32 ***	69.74