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. 2018 Nov 14;10(11):632. doi: 10.3390/v10110632

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Construction and characterization of recombinant Autographa californica multiple nucleopolyhedrovirus (AcMNPV) and the clonal cell lines. (A) Western blotting analysis of cell lines Sf9 and Sf^EGFP-Rab5 using an anti-EGFP antibody. An immunoreactive band of EGFP-LCa (51 kDa) was detected in the samples of Sf^EGFP-LCa, but not in the sample of the negative control Sf9. (B) One-step growth curves of cell lines Sf9 and Sf^EGFP-Rab5. Cells were infected with vAc^Pp10-EGFP at a MOI of 5. The titers of infectious budded virus were determined by TCID₅₀ endpoint dilution assays.