Table 1.
RT-PCR mixture.
| Reagent | Volume |
|---|---|
| DNase/RNase-Free Water | 6 μL |
| SYBR Green PCR Master mix (Toyobo) | 10 μL |
| Forward /Reverse primer (100 μM) | 0.8 μL |
| ROX Reference Dye | 0.4 μL |
| cDNA | 2 μL |
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