Figure 1.

Delayed treatment of infection using capsule depolymerases. (A) 1.2–3.4 × 10⁸ CFU of K1-capsulated E. coli treated with 20 μg of K1F or K1H enzyme; (B) 3.1–6.1 × 10⁸ CFU of K5-capsulated E. coli treated with 20 μg of K5 enzyme; (C) 1.1–2.8 × 10⁸ CFU of E. coli E69 treated with 20 μg of K30 enzyme. All inoculations were IM (intramuscular, thigh); enzyme was administered 8 h after bacteria in the contralateral thigh. Mouse survival was monitored for 5 days and Kaplan-Meier survival curves in solid lines were plotted with the cumulative probability of survival over time for each treatment. Previously reported survival curves of simultaneous enzyme treatments are included for comparison (dashed lines). The mouse number (n) of each treatment is given for each curve. Log rank test: p values are listed for delayed treatments compared to the no-enzyme control.