Figure 3.

In vitro activity comparison of depolymerases and cognate phages. 10–20 μg of K1 capsule were incubated with serial dilutions of K1E, K1F, K1H depolymerase or phages at the indicated amount of enzyme equivalents (0.25–8 μg). Active enzyme is indicated by loss of signal within the lane; enzyme associated with phages is 4–8 fold more active than free enzyme. Incubation was at 37 °C for 1 h; reactions were fractionated using 12% TBE-PAGE with Alcian Blue staining. Protein standards were loaded in the leftmost lane and their molecular weights indicated.