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Overexpression of type I toxins. (A) Normal growth was observed in MWprmc2 that contains an empty pRMC2 vector while complete growth inhibition was observed in MWprmc2354, MWprmc1888 and MWprmc1381 that contain Fst, TxpA, and holin toxins, respectively. (B) The bactericidal nature of three type I toxins was determined by ATC inducible plates, and the results showed that cells expressing Fst, TxpA, and holin toxins were unable to restore growth as compared to control MWprmc2.
