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. 2018 Nov 14;10(11):473. doi: 10.3390/toxins10110473

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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(A) Gel-shift assay was performed to detect the binding of toxin and antitoxin to the promoter. The up-shift represents the binding of the antitoxin protein to the 98 bp probe (lanes 5–7), while the toxin protein was unable to bind (lanes 2–4). No shift was observed when antitoxin and toxin proteins were mixed together with the 98 bp probe (lanes 8 and 9). (B) The TA genes MW1436-MW1437, and MW2329-M2330 were co-transcribed. 1 = marker, 2, 5 = RNA, 3, 6 = cDNA, and 4, 7 = DNA. The co-transcript product (lanes 3 and 4) represents the 700 bp size of MW1436-MW1437, and lanes 6 and 7 represent the 400 bp size of MW2329-MW2330.

(A) Gel-shift assay was performed to detect the binding of toxin and antitoxin to the promoter. The up-shift represents the binding of the antitoxin protein to the 98 bp probe (lanes 5–7), while the toxin protein was unable to bind (lanes 2–4). No shift was observed when antitoxin and toxin proteins were mixed together with the 98 bp probe (lanes 8 and 9). (B) The TA genes MW1436-MW1437, and MW2329-M2330 were co-transcribed. 1 = marker, 2, 5 = RNA, 3, 6 = cDNA, and 4, 7 = DNA. The co-transcript product (lanes 3 and 4) represents the 700 bp size of MW1436-MW1437, and lanes 6 and 7 represent the 400 bp size of MW2329-MW2330.