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. 2018 Jun 13;1(7):e57. doi: 10.1002/hsr2.57

Figure 4.

Figure 4

In vitro effect of dissolved oxygen (DO) on proliferation and cell death. Human skin fibroblasts (HSFs) were grown in DMEM with 1% FBS with (23‐50 mg/L) or without (11 mg/L) elevated levels of DO. DMEM with 10% FBS (11 mg/L) served as positive control. A) Treating HSFs with DO up to 50 mg/L (50 mg/L DO) did not significantly change the proliferation rate when compared with control. The positive control had significantly higher proliferation rate on day 3 and day 4 compared with control. Data are expressed as average ± SD (n = 4, *P < 0.05, ANOVA with Bonferroni post hoc test). B, Treating HSFs with DO up to 50 mg/L did not significantly alter the number of dead cells compared with control over the course of 4 days, with the exception of 31 mg/L at day 2. Triton X (not shown in figure) significantly increased the number of dead cells compared with control. Data are expressed as the percentage of dead cells relative to total cell number and represent the average ± SD (n = 4, *P < 0.05, ANOVA with Bonferroni post hoc test)