Detection of Listeria spp. strains employing the combined cell wall-binding domain-based magnetic separation (CBD-MS)/A511::luxAB assay. Dilutions of pure cultures of five different Listeria strains representing different serovars were used for evaluating the combined CBD-MS/A511::luxAB assay. (a) L. monocytogenes ScottA (serovar [SV] 4b); (b) L. monocytogenes EGDe (SV 1/2a); (c) L. monocytogenes WSLC 1001 (SV 1/2c); (d) L. ivanovii WSLC 3009 (SV 5); (e) L. innocua WSLC 2012 (SV 6b). The dotted horizontal bar indicates the level of background light emission (noise) of the assay, determined in the negative control sample (no bacteria). Asterisks indicate significant differences between values obtained from samples with bacteria and the respective control values. ****, p < 0.0001; ***, p < 0.001; **, p < 0.01; *, p < 0.05; ns, non-significant.