Table 4.
The effect of baby formula feeding on the copper metabolism of 9-day-old rats.
| Characteristics | Control | Experiment |
|---|---|---|
| Body weight, g | 11.8 ± 1.3 (11) | 9.4 ± 1.2 (11) |
| Serum Cp oxidase activity, mg/100 mL | 14.1 ± 2.13 (6) | 38.47 ± 4.46 (6) § |
| Serum Cp antigen activity, mg/100 mL | 14.3 ± 1.2 (6) | 43.2 ± 2.1 (7) § |
| Serum Cu concentration, μg/L | 300 ± 50 (6) | 940 ± 71 (7) |
| Cu atoms per 1 serum Cp molecule * | 4.5 | 4.5 |
| Cu content in liver, μg/g dry weight | 4.58 ± 0.04 (5) | 1.91 ± 1.20 (5) § |
| Cu content in brain, μg | 1.71 ± 0.40 (5) | 1.72 ± 0.61 (5) |
| 1 CSF Cp antigen activity, mg/100 mL & | 0.3 | 2.3 |
| Cu concentration in CSF, μg/L | 15 | 85 |
| Liver total RNA, mg/g tissue | 1.7 | 1.8 |
| Cp-mRNA, μg/mg of total RNA | 0.11 | 0.23 |
| Carbonyl group concentration, A370/mg protein: Cytoplasm Ψ of brain | 0.131 ± 0.02 (5) | 0.101 ± 0.013 (5) |
| Cytoplasm of liver | 0.093 ± 0.01 | 0.086 ± 0.07 |
| Serum | 0.155 ± 0.01 | 0.127 ± 0.014 |
Notes: In parentheses: number of animals; * calculated to immunoreactive Cp; & measurement made in mixtures from 3 µL aliquots of cerebrospinal fluid from rats of a group, simple mean from three measurements; Ψ supernatant obtained after sedimentation of tissue homogenate at 23,000× g 30 min; 1—cerebrospinal fluid, § p < 0.05.