TABLE 1.
Representative proteins associated with HDL in pregnant mice with different levels of apoA-I
| Protein | Peptide counta | ||
|---|---|---|---|
| apoA-I−/− | apoA-I+/+ | apoA-Itg/tg | |
| Complement C3 | 105.6 ± 5.7 | 82.5 ± 5.4 | 69.2 ± 2.9bc |
| Inter-α-trypsin inhibitor | 65.4 ± 5.5 | 61.5 ± 1.5 | 49.7 ± 3.6c |
| Serotransferin | 41.9 ± 3.1 | 45.3 ± 5.8 | 24.2 ± 4.1c |
| α-2-HS-glycoprotein | 27.6 ± 1.4 | 24.3 ± 1.7 | 26.5 ± 1.6 |
| Kininogen-1 | 25.5 ± 1.1 | 25.5 ± 2.0 | 19.3 ± 1.3 |
| Apolipoprotein A-I | 0.0 ± 0.0 | 45.2 ± 2.6 | 296.0 ± 24.0b |
| Pregnancy zone protein | 15.3 ± 1.2 | 9.1 ± 1.2 | 10.6 ± 1.9 |
| Hemopexin | 28.4 ± 1.1 | 25.1 ± 2.5 | 21.4 ± 2.6c |
| Murinoglobulin | 15.3 ± 2.3 | 14.4 ± 0.4 | 11.9 ± 0.9 |
| Vitamin D–binding protein | 24.8 ± 2.6 | 23.9 ± 2.0 | 19.4 ± 2.0 |
| Fibrinogen α chain | 18.2 ± 1.1 | 15.2 ± 4.4 | 12.6 ± 1.7 |
| Apolipoprotein A-IV | 13.7 ± 1.6 | 15.2 ± 1.4 | 21.0 ± 1.1c |
| Gelsolin | 14.1 ± 5.4 | 12.9 ± 1.9 | 16.9 ± 2.3 |
| Plasminogen | 14.9 ± 3.1 | 14.1 ± 2.2 | 10.5 ± 0.8 |
| α-1-B glycoprotein | 16.8 ± 2.6 | 19.0 ± 1.0 | 13.6 ± 1.7 |
| Prothrombin | 20.1 ± 1.6 | 17.9 ± 0.4 | 14.3 ± 2.3c |
| Afamin | 7.9 ± 1.0 | 12.5 ± 2.3 | 10.9 ± 1.0 |
| Complement factor B | 17.3 ± 0.6 | 14.1 ± 0.7 | 10.9 ± 1.2bc |
| Fibronectin | 10.5 ± 3.0 | 3.0 ± 0.4 | 6.5 ± 3.1 |
| Leukemia inhibitory factor receptor | 21.9 ± 4.8 | 24.3 ± 1.0 | 14.3 ± 0.7 |
| Clusterin | 13.7 ± 1.7 | 8.8 ± 1.7 | 8.1 ± 1.1 |
| Complement factor H | 10.7 ± 1.3 | 7.2 ± 1.0 | 4.8 ± 0.4bc |
| Complement C4-B | 11.4 ± 0.7 | 8.0 ± 1.1 | 6.1 ± 1.1bc |
| Fibrinogen β chain | 11.7 ± 1.8 | 9.1 ± 0.1 | 8.8 ± 0.3 |
| Paroxonase | 8.5 ± 0.8 | 11.8 ± 0.4 | 7.1 ± 2.0 |
| Ceruloplasmin | 13.0 ± 1.9 | 15.6 ± 2.4 | 11.6 ± 0.3 |
| Inter-α-trypsin inhibitor heavy chain H2 | 8.3 ± 1.3 | 6.5 ± 2.1 | 7.4 ± 1.4 |
| Apolipoprotein E | 18.2 ± 0.9 | 10.6 ± 1.7 | 1.7 ± 0.7bc |
| Corticosteroid-binging globulin | 8.8 ± 0.9 | 10.6 ± 2.1 | 11.9 ± 1.2 |
| Fibrinogen γ chain | 9.3 ± 0.7 | 5.7 ± 1.8 | 5.2 ± 0.6 |
| Complement factor I | 5.4 ± 0.4 | 5.3 ± 1.4 | 6.1 ± 0.7 |
| Hemoglobin subunit β-1 | 5.0 ± 2.1 | 4.6 ± 1.3 | 10.7 ± 0.7c |
| α-1 antitrypsin 1 | 6.8 ± 1.1 | 8.0 ± 0.7 | 5.8 ± 0.7 |
| Carboxypeptidase N subunit | 9.5 ± 1.3 | 8.7 ± 1.0 | 4.4 ± 0.3bc |
| Hemoglobin subunit α | 6.4 ± 0.6 | 6.1 ± 2.7 | 3.4 ± 0.9 |
| Inter-α-trypsin inhibitor heavy chain H1 | 7.1 ± 1.1 | 5.7 ± 0.7 | 4.4 ± 0.8 |
| Fetuin-B | 5.8 ± 1.2 | 3.8 ± 0.8 | 3.7 ± 0.6 |
| Inhibitor of carbonic anhydrase | 4.1 ± 0.9 | 3.0 ± 1.0 | 5.8 ± 0.6 |
| Carboxyesterase | 3.7 ± 0.5 | 1.9 ± 0.4 | 5.4 ± 0.3 |
| β-2-glcoprotein 1 | 3.7 ± 0.5 | 3.4 ± 0.7 | 2.0 ± 0.6 |
| Inter-α-trypsin inhibitor heavy chain H3 | 5.1 ± 1.7 | 3.4 ± 0.7 | 2.7 ± 0.4 |
| Vitronectin | 5.9 ± 0.7 | 3.8 ± 1.0 | 1.0 ± 0.1bc |
| Complement C5 | 4.5 ± 0.8 | 4.2 ± 1.3 | 1.4 ± 0.3bc |
| Antithrombin-III | 3.4 ± 0.3 | 2.3 ± 0.7 | 0.7 ± 0.7bc |
Data are presented as means ± sem (n = 3). aNumber of peptides identified by mass spec fragmentation per protein. bUnadjusted significant difference between apoA-I−/−/apoA-I+/+/apoA-Itg/tg. cUnadjusted significant correlation with apoA-I (P < 0.05).