TABLE 1.

Mouse primers used for real-time qPCR and ChIP-qPCR

	Primer, 5′–3′
Gene	Forward	Reverse
ZAG	GGACACTACAGGGTCTCACACCTT	TGAAATCCTCTCCGTCGTAGGC
Ap2	ACACCG AGATTTCCTTCAAACTG	CCATCTAGGGTTATGATGCTCTTCA
CideA	TGCTCTTCTGTATCGCCCAGT	GCCGTGTTAAGGAATCTGCTG
Dio2	CAGTGTGGTGCACGTCTCCAATC	TGAACCAAAGTTGACCACCAG
Pgc1α	CCCTGCCATTGTTAAGACC	TGCTGCTGTTCCTGTTTTC
SRC1	TTTCAAGAAGTGATGACTCGTGG	CCAGGATTGACTGAGGGATT
Pparγ	GTGCCAGTTTCGATCCGTAGA	GGCCAGCATCGTGTAGATGA
Prdm16	CAGCACGGTGAAGCCATTC	GCGTGCATCCGCTTGTG
Ebf2	GCTGCGGGAACCGGAACGAGA	ACACGACCTGGAACCGCCTCA
Ucp1	ACTGCCACACCTCCAGTCATT	CTTTGCCTCACTCAGGATTGG
Elovl3	TCCGCGTTCTCATGTAGGTCT	GGACCTGATGCAACCCTATGA
Nrf1	GAACTGCCAACCACAGTCAC	TTTGTTCCACCTCTCCATCA
Nrf2	GCTTTTGGCAGAGACATTCC	ATCAGCCAGCTGCTTGTTTT
Essrα	TTCTGCACAGCTTCCACATC	GGAAGAATTCGTCACCCTCA
Cycs	GCAAGCATAAGACTGGACCAAA	TTGTTGGCATCTGTGTAAGAGAATC
Cpt1b	CGAGGATTCTCTGGAACTGC	GGTCGCTTCTTCAAGGTCTG
Cox3	GCAGGATTCTTCTGAGCGTTCT	GTCAGCAGCCTCCTAGATCATGT
Cox4	ACCAAGCGAATGCTGGACAT	GGCGGAGAAGCCCTGAA
Cox5b	GCTGCATCTGTGAAGAGGACAAC	CAGCTTGTAATGGGTTCCACAGT
Cox7a	CAGCGTCATGGTCAGTCTGT	AGAAAACCGTGTGGCAGAGA
Cox8b	GAACCATGAAGCCAACGACT	GCGAAGTTCACAGTGGTTCC
CD40	TTGTTGACAGCGGTCCATCTA	CCATCGTGGAGGTACTGTTTG
Tnfrsf9	CGTGCAGAACTCCTGTGATAAC	GTCCACCTATGCTGGAGAAGG
Tbx1	GGCAGGCAGACGAATGTTC	TTGTCATCTACGGGCACAAAG
Tmem26	ACCCTGTCATCCCACAGAG	TGTTTGGTGGAGTCCTAAGGTC
Mouse 18S rRNA	GTAACCCGTTGAACCCCATT	CCATCCAATCGGTAGTAGCG
Prdm16-ChIP	GACCTCCTGCCTTCCCTGAGG	GCTGCCTGAGCTGGGCCAGCC
Ucp1-ChIP	AGTGAAGCTTGCTGTCACTC	GTCTGAGGAAAGGGTTGACC
18s-ChIP	AGTCCCTGCCCTTTGTACACA	CGATCCGAGGGCCTCACT

Elovl3, ELOVL fatty acid elongase 3; Nrf1/Nrf2, nuclear respiratory factor 1/2; Esrrα, estrogen-related receptor α; Cycs, cytochrome c, somatic; Cpt1b, carmitine palmitoyltransferase 1b; Tnfrsf9, TNF receptor superfamily member 9; Tbx1, T-box 1; Tmem26, transmembrane protein 26.