Figure 1.

In vivo leukocyte transmigration rate in IL-1β-implanted mouse cornea. A) In vivo imaging assay for quantification of leukocyte transmigration rate. B) Pellets containing IL-1β were implanted in the corneas of BALB/c mice. Composite photomicrographs of corneal neovascularization. Double staining of corneal flatmounts for leukocytes (CD45) and angiogenesis (ConA). AO⁺ traveling leukocytes in IL-1β-implanted corneas. Zone separation by the distance (300 µm) from the limbal vessels. To depict the corneal limbal vessels and the corneal stroma, including the site of pellet implantation at high resolution, adjacent micrographs were taken and merged in a mosaic fashion. Asterisk: implanted pellets. Scale bars, 100 µm.