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. 2017 Sep 19;32(1):440–452. doi: 10.1096/fj.201700485RR

Figure 1.

Figure 1.

Ocy454 subclone characterization. Ocy454 clones: 3 low- (9-Low, 4-Low, and 24-Low) and 2 high-sclerostin–expressing clones (15-High and 12-High) were used for additional characterization. A–C, E, H) Semiquantitative PCR Sost (A), Dmp1 (B), Phex (C), Mef2c (E), and CAIII (H) are significantly increased in 2 high (15-High and 12-High; red bars) clones compared with 3 low (9-Low, 4-Low and 24-Low; black bars) clones after 7 (solid bars) and 14 (hatched bars) d in culture (regular medium, 37°C). D, F, G) Hdac7 (F) and E11/gp38 (G) expression is significantly lower in 15- and 12-High compared with 9-, 4-, and 24-Low, whereas RANKL (D) is unchanged across subclones. Gene expression is normalized to β-actin. Data are expressed as means ± sd of triplicates. Each experiment was repeated at 3–4 times. Two-way ANOVA with Tukey’s multiple comparison test was performed. *P < 0.05, **P < 0.01.