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. 2017 Sep 19;32(1):440–452. doi: 10.1096/fj.201700485RR

Figure 2.

Figure 2.

Transcripts that are differentially regulated in high- and low-expressing clones. A–C) Semiquantitative PCR for Srpx2 (A), CD200 (B), and DKK1 (C). These transcripts are significantly increased in and 2 high (15-High and 12-High; red bars) clones compared with 3 low (9-Low, 4-Low and 24-Low; black bars) clones after 7 d of differentiation at a nonpermissive temperature (37°C). D–H) Aspn (D), Enpp2 (E), Nov (F), Robo2 (G), and Serpina3g (H) are significantly lower in 15- and 12-High compared with 9-, 4-, and 24-Low. Gene expression is normalized to β-actin. Data are expressed as means ± sd of triplicates. Each experiment was repeated at 3–4 times. One-way ANOVA with Tukey’s multiple comparison test was performed. **P < 0.01.