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. 2017 Sep 22;32(1):488–499. doi: 10.1096/fj.201700670R

TABLE 1.

PCR primer sequences and amplification parameters

Gene Primer sequence, 5′–3′
Product size (bp) Temperature (°C)
Sense Antisense
β-Actin GGTCATCACTATTGGCAACGAG GAGGTCTTTACGGATGTCAACG 142 57
MAFbx TCAGAGAGGCAGATTCGCAA TCCAGGAGAGAATGTGGCAG 154 59
MuRF1 TTTGACACCCTCTACGCCAT TTGGCACTTGAGAGGAAGGT 203 59
PHD1 TGTCACTGTGGTGTTTGGCTAC GCATTTATCAGGATGGGAAGG 123 61
PHD2 CCTGCCATTGGTGATAGTGAC GGGTGGAAGGGTAAGAAACAT 153 63
PHD3 TCAAGGCTGTGAGGTAGTCT CTTGCATGGGAGGCTCATC 186 61
ADRB2 TCAAGGCTGTGAGGTAGTCT CTTGCATGGGAGGCTCATC 192 59