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. 2018 Nov 23;9:2752. doi: 10.3389/fmicb.2018.02752

FIGURE 7.

FIGURE 7

LB supplementation with octanoate increased the pyocyanin production in all analyzed strains, but nfxB∗ remained producing lower levels than those observed in PAO1 when grew in LB medium. For pyocyanin assay, the strains were grown in LB medium with or without octanoate (5 mM) along a time lapse of 20 h and the pyocyanin was extracted with chloroform-based protocol as is described in Methods. Statistical significances were evaluated by using a Student’s two-tailed test and considered significant if P < 0.05, with a confidence interval of 95% (∗P < 0.05; ∗∗P < 0.01; ∗∗∗P < 0.001). The results show that supplementation of LB with 5 mM octanoate, even allowing nfxB∗ strain increase the pyocyanin production, was insufficient to reach the levels produced by PAO1 in LB without octanoate. This indicate that a hypothetical low intracellular accumulation of octanoate is not the main cause for defective pyocyanin production observed in nfxB∗ strain.