Table 1.
Bacterial strains and plasmids used in the present work.
| Bacterial strain/plasmids | Description | Reference/origin |
|---|---|---|
| Escherichia coli | ||
| One Shot OmniMaxTM 2 T1 | Host strain used for the maintenance of cloning plasmids: F′ proAB lacIq lacZΔM15 Tn10(TetR) Δ(ccdAB) mcrA, Δ(mrr,hsdRMS-mcrBC) ϕ80(lacZ)ΔM15 Δ(lacZYA-argF)U169 endA1 recA1 supE44 thi-1 gyrA96 relA1 tonA panD | Invitrogen |
| S17-1λ pir | Conjugative donor strain used for transferring plasmids to P. aeruginosa acceptor strains by conjugation assays: F− thi pro hsdR hsdM+ recA RP42-Tc::Mu-Km::Tn7 | Simon et al., 1986 |
| S17 miniCTX::PpqsA-lux | S17-1λ pir strain containing the miniCTX::PpqsA-lux plasmid | Fletcher et al., 2007a,b |
| JM109-pSB1142 (LasR-based Biosensor) | Biosensor strain used for detecting the QS signal, 3-oxo-C12-HSL, produced by P. aeruginosa strains | Winson et al., 1998 |
| JM109-pSB536 (RhlR-based Biosensor) | Biosensor strain used for detecting the QS signal, C4-HSL, produced by P. aeruginosa strains | Swift et al., 1997 |
| Pseudomonas aeruginosa | ||
| PAO1 | Wild-type PAO1-V clinic strain given from the lab of V. de Lorenzo | Linares et al., 2005 |
| PAO1 miniCTX::PpqsA-lux (PAO1_PpqsA) | PAO1-V strain with the reporter construction PpqsA-luxCDABE inserted in the specific attB site of the chromosome | Present work |
| JFL28 (nfxB∗) | Spontaneous resistant mutant obtained from PAO1-V strain, which overproduces the MexCD-OprJ efflux system by punctual inactivating mutation in nfxB gene | Linares et al., 2005 |
| JFL28 miniCTX::PpqsA-lux (nfxB∗_PpqsA) | JFL28 strain with the reporter construction PpqsA-luxCDABE inserted in the specific attB site of the chromosome | Present work |
| nfxB∗ΔmexD | JFL28 strain with an inactive MexCD-OprJ efflux system by partial deletion of the mexD gene | Present work |
| PAO1 CTX::PpqsA-lux::pqsA (PqsR-based Biosensor) | PAO1-ΔpqsA strain with the reporter construction PpqsA-luxCDABE inserted in the specific attB site of the chromosome. Used for detecting the AQs produced by other P. aeruginosa strains | Fletcher et al., 2007a,b |
| Plasmid | ||
| pGEM-T Easy | Commercial plasmid used for cloning optimization of PCR products (AmpR) | Promega |
| pGEM-T-ΔmexD | pGEM-T Easy vector with the flanking DNA sequences of a 2058 bp inner region of mexD gene (AmpR) | Present work |
| pEX18Ap | Plasmid with conjugative properties used for deleting genes in P. aeruginosa by homolog recombination. AmpR | Hoang et al., 1998 |
| pEX18Ap-ΔmexD | pEX18Ap vector with the flanking DNA sequences of a 2058 bp inner region of mexD gene used for deleting mexD gene in P. aeruginosa strains. AmpR | Present work |
| Mini-CTX-lux-PpqsA | Plasmid derived from mini-CTX-lux Becher and Schweizer (2000) in which the expression of the luxCDABE operon is under the transcriptional control of the pqsABCDE promoter region of P. aeruginosa. TcR | Fletcher et al., 2007a,b |
| pSB1142 | Plasmid carried by the LasR-Bioreporter strain necessary for detecting 3-oxo-C12-HSL. TcR | Winson et al., 1998 |
| pSB536 | Plasmid carried by the RhlR-Bioreporter strain necessary for detecting C4-HSL. AmpR | Swift et al., 1997 |