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. 2018 Nov 23;8:411. doi: 10.3389/fcimb.2018.00411

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Copyright © 2018 Brock, Haselkorn, Garcia, Bashir, Douglas, Galloway, Brodie, Queller and Strassmann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Venn diagram of soil and feces bacteria genera associated with D. discoideum. We found seven bacteria genera overlap between soil and feces environments. We identified about twice as many culturable bacteria genera associated with D. discoideum isolated from feces compared to soil environments. A 460 bp PCR fragment generated from 16S rRNA primers was sequenced to identify these bacteria genera. The asterisk signifies bacteria genera of equal sequence identity.