Table 2.
Primers used for obtaining pR’ and rfp fragments.
Primer | Sequence (5′-3′) a) | Restriction Site |
---|---|---|
LP F1-1 | 5′-CGGGAAGGTACCACCTCTGTATTTTATCAG-3′ | KpnI |
LP R1-3 | 5′-GGGCCGTCTAGAAAAGAAAAAAGTTAGCAC-3′ | XbaI |
LP F2-2 | 5′-ATTAGTCCCGGGCTTGGATTTATTGATGGT-3′ | SmaI |
LP R3-2 | 5′-ATAACGTCTAGATAACAGGCACAGTACCCA-3′ | XbaI |
LP F3-2 | 5′-AGCGGTACCAAAAACCGGAAACGTGTA-3′ | KpnI |
LP F4-1 | 5′-TGCGTAGGTACCAGCGTCTATAATTGTATG-3′ | KpnI |
LP R4-2 | 5′-GCATTATCTAGACAACAGGCACAGTATCCA-3′ | XbaI |
RFP F-2 | 5′-CTGATATCTAGAATGGCCTCCTCCGAG-3′ | XbaI |
RFP R-5 | 5′-ATCTGTAAGCTTCTACAGGAACAGGTGGT-3′ | HindIII |
a) Restriction enzyme sites are underlined.