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. 2018 Nov 23;9:2810. doi: 10.3389/fmicb.2018.02810

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Copyright © 2018 Kim, Kim, Kim, Hwang and Yoon.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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OMVs-associated T3SS1 effectors are located on the outer surface of OMVs. OMVs were isolated from cultures of Salmonella strains producing HA-tagged SipA, SipB, SipC, SopA, SopB, SopE2, or SseD. OMVs treated or untreated with proteinase K were precipitated with trichloroacetic acid (TCA) and subsequently analyzed by immunoblotting using anti-HA antibody. SseD protein located in the lumen of vesicles and an integral membrane protein OmpA of OMVs were used as controls.