Gene cloning of Latroeggtoxin-V. (A) Identification of the total RNA integrity with agarose gel electrophoresis. M, DNA marker; Lane 1, total RNA. (B) Nucleic acid sequence encoding Latroeggtoxin-V and the corresponding amino acid sequence. The signal sequence is shaded in gray, followed by the mature peptide sequence of Latingtoxin-V. The asterisk indicates the stop codon. (C) Detection of PCR product of Latroeggtoxin-V gene with agarose gel electrophoresis. M, DNA marker; Lane 1, PCR product, a 310-bp nucleic acid sequence containing the 276-bp gene fragment and restriction endonuclease and enterokinase cleavage site sequences.