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. 2018 Nov 2;10(11):451. doi: 10.3390/toxins10110451

Table 2.

Primer sequences.

Primer Name Primer Sequence (5′→3′)
Latroeggtoxin-V-F CATGGGATCCGACGACGACGACAAG=TCTCAGGCTGGTGAATGGGGTTCTG
Latroeggtoxin-V-R CCGCTCGAGTTAACCGTCGATTTTTTTTTCTTCTTTAGC

Notes: The single underline indicates restriction endonuclease cleavage sites, whereas the double underline indicates enterokinase cleavage site. GGATCC and CTCGAG are cleavage sites of BamHI and XhoI, respectively.