Figure 8.

Mitochondrial oxidative stress contributes to bystander induced inflammatory response. (a) Experimental workflow indicating U-87 MG astrocytes were pre-treated with either BAY-82, MitoQ, or the DMSO solvent control prior to inoculation with TC-83 (MOI:2). Supernatants were removed from infected astrocytes at 1 hpi and overlaid onto BV2 microglia, with inflammatory cytokine production being measured at (b) 1 h and (c) 6 h post overlay. The quantitative data are depicted as the means of three biologically independent experiments ± SD. * indicates significant difference (p < 0.05) between the indicated sample and the time-matched DMSO control.