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Gating strategy for Figure 3d. (a) Uninfected and (b) TC-83 infected (MOI:2) BV2 microglia and were collected at 2 hpi and fixed using paraformaldehyde. Cells were stained with phycoerythrin-conjugated Iba1 (PE-A) as a positive control for selection of microglial cells. A phycoerythrin-cyanin5-conjugated CD11b (PE-Cy5-A) antibody was used to determine microglial activation in the context of TC-83 infection. Unstained cells were maintained as a negative control for fluorescence.
