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AAV8 Glycosylation detection. AAV8 derived from the cell (intracellular), and media (secreted) are separated on SDS-PAGE (a) protein bands visualized after glycoprotein staining. Glycosylated bands were observed near the AAV8 capsid protein (VP) region (55–100 kDa). Gel (b) is gel (a) stained with Coomassie brilliant blue to visualize the whole protein profile. (c) Independent Coomassie stained gel run after de-N- glycosylation of secreted sample. (+ and − the addition of PNGase F).
