Figure 6.
The 2× direct plasma treatments reduced the expression of the proliferative marker ki67 in glioblastoma spheroids. The 2× direct plasma treatment decreased the % ki67+ cells in (a) U87-Red spheroids (p < 0.05) and was lower than in those untreated or treated with pPBS and RONS mix (p < 0.05). (b) The same trend was observed in U251-Red spheroids (p > 0.05). The staining was scored using IHC Profiler in ImageJ. (c) Representative images of ki67 staining of U87-Red and U251-Red spheroids exposed to untreated PBS or He + 20% H2O direct treatment. Enlarged image demonstrates variable ki67 staining. No ki67 staining control on the right. Data representative of two independent experiments, 4–6 spheroids per condition. Mean ± SD; * = p < 0.05. Scale bar = 500 µm.