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. 2018 Oct 27;10(11):589. doi: 10.3390/v10110589

Figure 4.

Figure 4

Transmission of SmEV1 in strain LC22 of Sclerotinia minor through sclerotia. (A) A histograph showing the average lesion diameters caused by the hypovirulent strain LC22, 104 single-sclerotium (SS) isolates of strain LC22 and the virulent strain LC41 on detached leaves of oilseed rape (20 °C, 72 h). Note reduced average leaf lesion diameters caused by strain LC22 and its SS progenies, compared to the lesion caused by LC41. Eight representative SS isolates selected for further analyses were labeled in the graph; (B) Colony morphology of LC22, eight SS isolates and LC41 (PDA, 20 °C, 30 d); (C) Average growth rates (n = 5) for LC22, eight SS isolates and LC41. Different letters on the bars in each graph indicate significant difference (p < 0.05) according to the Least Significant Difference test; (D) Average sclerotial yield (n = 5) produced by LC22, eight SS isolates and strain LC41 in 30-day-old PDA cultures; (E) Detection of SmEV1 in LC22, eight SS isolates and LC41 by dsRNA extraction and RT-PCR.