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. 2018 Oct 27;10(11):589. doi: 10.3390/v10110589

Figure 5.

Figure 5

Effect of pre-treatment of oilseed rape leaves with the hyphal fragment suspension (HFS) of the hypovirulent strain LC22 of Sclerotinia minor on infection by the virulent strain LC41 of S. minor. (A) Lesions caused by strain LC41 at 96 h post inoculation (20 °C) on leaves of different treatments. PDB + Agar, the leaf was pre-treated with potato dextrose broth (PDB) alone and challenge-inoculated with a fresh PDA plug; HFS + Agar, the leaf was pre-treated with the HFS of LC22 alone and challenge-inoculated with a fresh PDA agar; PDB + LC41, the leaf was pre-treated with PDB alone and challenge-inoculated with a mycelial agar plug of LC41; HFS + LC41, the leaf was pre-treated with the HFS of LC22 and challenge-inoculated with a mycelial agar plug of LC41; (B) A histograph showing average leaf lesion diameters (n = 18) of different treatments. ** p < 0.01, Student’s t test.