Table 1.
Endornavirus SmEV1 transmission from the donor strain LC22 (MCG 5) to other strains of Sclerotinia minor (MCGs 1 to MCG 11), and effect of SmEV1 introduction on mycelial growth, sclerotial yield, and pathogenicity of the recipients.
| Recipient Strain (MCG) | SmEV1 1 | Growth Rate (cm/d) 2 | Sclerotia Per Dish 2 | Leaf Lesion Diameter (cm) 3 | ||||
|---|---|---|---|---|---|---|---|---|
| Before | After | Before | After | Before | After | Before | After | |
| LC53 (MCG1) | − | + | 1.8 | 0.6 ** | 1806 | 1259 ** | 4.5 | 1.6 ** |
| XN19 (MCG1) | − | + | 1.8 | 0.7 ** | 2286 | 835 ** | 3.3 | 2.2 ** |
| XN21 (MCG1) | − | + | 1.9 | 0.5 ** | 1881 | 674 ** | 3.6 | 0.4 ** |
| XN35 (MCG1) | − | + | 1.6 | 0.2 ** | 2550 | 1200 * | 2.9 | 0.5 ** |
| DY02 (MCG2) | − | + | 1.5 | 1.0 ** | 1978 | 1411 ** | 3.7 | 1.6 ** |
| LC02 (MCG2) | − | + | 1.7 | 1.1 ** | 2300 | 843 ** | 3.6 | 1.6 ** |
| LC20 (MCG2) | − | + | 1.5 | 0.7 ** | 1719 | 734 ** | 3.1 | 1.0 ** |
| LC28 (MCG2) | − | + | 1.4 | 1.1 ** | 2416 | 1278 * | 3.8 | 0.9 ** |
| LC41 (MCG2) | − | + | 1.8 | 1.2 ** | 2198 | 1196 ** | 3.2 | 0.5 ** |
| LC11 (MCG3) | − | + | 1.6 | 0.3 ** | 2224 | 575 ** | 3.3 | 1.0 ** |
| LC19 (MCG3) | − | + | 1.7 | 0.2 ** | 1742 | 1014 ** | 3.2 | 0.6 ** |
| LC46 (MCG3) | − | + | 1.7 | 0.9 ** | 2432 | 889 ** | 2.6 | 0.8 ** |
| XN01 (MCG3) | − | + | 1.8 | 0.8 ** | 2880 | 1673 ** | 2.5 | 0.6 ** |
| XN12 (MCG4) | − | + | 2.0 | 1.2 ** | 2792 | 1408 ** | 3.9 | 0.2 ** |
| XN13 (MCG4) | − | + | 1.6 | 1.1 ** | 2556 | 1000 ** | 3.5 | 2.4 ** |
| LC15 (MCG5) | − | + | 1.8 | 1.4 ** | 2316 | 1475 ** | 3.4 | 1.3 ** |
| LC38 (MCG5) | − | + | 1.3 | 0.3 ** | 1188 | 674 ** | 0.8 | 0.2 ** |
| XN14 (MCG6) | − | + | 1.7 | 1.4 ** | 2350 | 1225 ** | 2.2 | 1.3 ** |
| XN34 (MCG6) | − | + | 1.8 | 0.4 ** | 1661 | 1124 * | 4.0 | 0.7 ** |
| LC29 (MCG7) | − | + | 2.0 | 0.8 ** | 2492 | 1105 ** | 3.8 | 0.7 ** |
| LC47 (MCG7) | − | + | 2.1 | 1.2 ** | 2459 | 1472 ** | 3.5 | 1.1 ** |
| LC36 (MCG8) | − | + | 1.5 | 0.6 ** | 2691 | 1152 ** | 2.9 | 2.1 ** |
| P13 (MCG9) | − | + | 1.9 | 0.3 ** | 1029 | 528 ** | 3.5 | 0.6 ** |
| W1 (MCG10) | − | + | 1.7 | 0.2 ** | 1788 | 782 ** | 3.7 | 0.7 ** |
| W26 (MCG11) | − | + | 1.6 | 0.3 ** | 1230 | 956 ** | 3.4 | 0.7 ** |
1 Based on detection of SmEV1 RdRp domain using RT-PCR tests for each strain three times with independent total RNA extractions. “+” and “−” indicate presence and absence of SmEV1, respectively; “*” and “**” indicate significant difference at p < 0.05 and p < 0.01, respectively, according to Student’s t test. 2 Average growth rate and the sclerotial yield values for each S. minor strain of 10 replicates (two tests each with 5 replicates) on PDA at 20 °C in Petri dishes (9 cm in diameter). 3 Average of lesion diameter of detached leaves assays of oilseed rape with 12 replicates (two tests each with three leaves, each leaf with two inoculation sites).