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. 2018 Oct 30;10(11):592. doi: 10.3390/v10110592

Figure 2.

Figure 2

Infection of 293T cells with CHIKV-mCherry in the presence of 50 nM silvestrol. (A) 293T cells were seeded in 24-well plates and 50 nM silvestrol was added. Afterwards, cells were infected with CHIKV-mCherry using an MOI of 3. Viral replication was determined at the indicated time points by flow cytometry detecting mCherry. The data are mean values of three experiments carried out in triplicate. (−) Untreated cells; (+) silvestrol-treated cells; (B) Western blot analysis of cell lysates prepared from 293T cells infected with CHIKV-mCherry as described above. The mCherry-nsP3 fusion protein was detected with an antibody directed against mCherry and the ECL detection system (Amersham, Freiburg); (C) Detection of the CHIKV-nsP2 protein; and, (D) Detection of the CHIKV-E2 protein; (E) Detection of β-actin as the loading control.