Table 1.
Characteristics of subjects enrolled in the study
| Variable | HD | LTBI | TB | TB-HIV | Total |
|---|---|---|---|---|---|
| Total subjects, n | 26 | 14 | 24 | 5 | 69 |
| Age (range) | a) | 34–60 | 22–38 | 28–40 | |
| Clinical characteristics | a) | No disease, | Pulmonary TB (22)Miliary TB (1) | Miliary TB and | |
| Mantoux positive | Extra-pulmonary TB (1) | HIV co-infection | |||
| Miliary TB (1) | |||||
| Nationality | b) | Italian | Italian (6) | Italian (2) | |
| North African (18) | North African (3) | ||||
| Subjects used for ex vivo analysis, n | 26/26 | 14/14 | 24/24 | 5/5 | 69 |
| Subjects included in ex vivo analysis of memory compartment (TM+ events count >100) | 16/26 | 11/14 | 20/24 | 5/5 | 52 |
| Subjects used for expansion with PHA and HLA-E peptides | 26/26 | 12/14 | 17/24 | 4/5 | 60 |
| Subjects included in functional analysis upon expansion (TM+ events count >1000) | 14/26 | 6/13 | 15/17 panel A 14/17 panel B |
- | 35 |
| Subjects used for expansion with PHA and HLA-A2 peptides | 3/26 | 3/13 | 7/17 panel A | - | 13 |
| 6/17 panel B | 12 | ||||
| Subjects used for TCR Vβ16 analysis | 6/26 | 7/13 | 8/17 | - | 21 |
a)
No information of the anonymous samples was available.
b)
13 samples were obtained from anonymous bloodbank donors (Sanquin Bloodbank, the Netherlands); 13 samples were collected from the Dipartimento di Medicina Clinica e delle Patologie Emergenti, University Hospital, Palermo.
HD, Healthy Donor; LTBI, Latently TB Infected subjects; TB, active TB patients TB-HIV, TB-HIV co-infected patients.