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. 2018 Oct 31;10(11):597. doi: 10.3390/v10110597

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Schematic representation for the generation of rZIKV: Mammalian Vero cells are transiently transfected with infectious RNA transcribed in vitro from a full-length cDNA clone (top), full-length infectious cDNA clones (left) or infectious subgenomic amplicons (right) (see also Figure 2). After 3–4 days post-transfection, when cytopathic effect is observed, rZIKVs present in tissue-culture supernatants can be recover for titration or viral amplification in mammalian (Vero, left) or insect (C6/36, right) cells.