Figure 3.

In vitro cytotoxic effect of CD, SMA–CD and Fe–Cur₃, on hormone-responsive (MCF-7) and hormone resistant breast cancer cells (MDA-MB-231 and 4T1). (A) MCF-7, (B) MDA-MB-231, and (C) 4T1 cells were treated with different concentrations of CD and SMA–CD. (D) MDA-MB-231 cells were treated with Fe–Cur₃. All cells were incubated for 48 h at 37 °C, 5% CO₂. Control wells were treated with vehicle only (0.2% dimethyl sulfoxide (DMSO)). At the end of treatment, the cell number was determined using the sulforhodamine B assay. Data are expressed as mean ± SEM (n = 3). Nonlinear regression and IC₅₀ value determination was performed using GraphPad Prism 6. Differences were considered significant at p < 0.05.