Fig. 1.

DIA MS workflow and analysis. (a) A spectral library was generated using different types of samples. Immortalized human fetal astrocytes were incubated with TNF (100 ng/ml), IL-1β (100 ng/ml), and LPS (10 μg/ml) for 24 h, and protein samples were analyzed using DIA LC-MS. Quantitative data were extracted by matching DIA MS data with the spectral library. Significantly changing proteins (FDR < 0.05, |FC| > 1.2) were selected after statistical analysis. (b) Venn diagram displaying significantly changing proteins for each group. (c) Principal component analysis (PCA) and hierarchical clustering (d) on the abundances of proteins significantly changing in at least one condition